Feline rhinotracheitis, calicivirus disease, and panleukopenia are common diseases in cats, which bring great pain to pets and great distress to owners; the infection rates of these three viruses are all At more than 30%, they have become the three most important viral infectious diseases that endanger the health of pet cats. They are very harmful to cats and are prone to mixed infections. Prevention and control are particularly important. Therefore, the cat triple vaccine is basically the only vaccine for pet cats. A must-have immune breed.

The triple inactivated vaccine is a safe and effective vaccine to prevent feline rhinotracheitis, calicivirus disease, and panleukopenia. Vaccination can help cats build immunity and reduce the risk of infection. This vaccine not only protects the health of cats, but also protects humans from infection.

The size of my country's pet vaccine market in 2022 will be 7.6 billion yuan, a year-on-year increase 17%. Driven by the continued prosperity of the "cat economy", the cat vaccine market will reach 3.4 billion yuan in 2022, a year-on-year increase of 30.1%, accounting for 45.5%; among which, the cat triple vaccine market can reach 2.78 billion yuan, accounting for 80.7% of the cat vaccine market .

Purification process of cat triple vaccine

Inactivated vaccine is a type of vaccine that is inactivated by physical or chemical treatment after culturing viruses or bacteria to obtain non-infectious activity. Compared with attenuated vaccines and genetically engineered vaccines, inactivated vaccines have the advantages of short development cycle, relatively mature preparation process, non-infectious virulence and high safety. The production process of inactivated vaccines mainly includes five steps: pathogen cultivation, pathogen inactivation, pathogen extraction and purification, vaccine preparation and packaging, and vaccine quality control. This article mainly introduces the downstream purification scheme of cat triple vaccine. .

After the virus harvest liquid is concentrated by ultrafiltration, use Bojin Biotech’s special virus purification medium MixQ400S/700S through flow-through mode or use large-pore GPQ-60 (1000) strong anion exchange medium through binding washing Off-mode solves the purification of three viruses. The amount of supernatant processed in a single batch is large, and the SDS-page purity of impurities after purification is less than 5%, and the impact on virus yield and titer is low.

Part of the data display of the experiment:

博For three types of viruses, FPV (20-30nm in diameter), FCV (20-30nm in diameter), and FHV (about 100nm in diameter), we selected composite mode media with corresponding 400kDa and 700kDa cutoff ranges for experiments.

Summary of the final impurity removal effect and the impact on virus yield:

From the experimental results, for two viruses FPV and FCV with a diameter of 20-30nm, Bojin Biotech’s MixQ400S has a good impurity removal effect, and MixQ400S/700S has a good impurity removal effect for viruses around 100nm FHV; Bojin Biotechnology's special medium for virus purification has less impact on virus yield in flow-through mode.